ELISA kit for PTX3 measurement

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What is Pentraxin3（PTX3/TSG-14)?

PTX3, a member of the pentraxin family, is a long pentraxin.
The short pentraxin CRP belonging to the pentraxin family that is produced in the liver is a known arteriosclerosis marker and is elevated during infection. Therefore, CRP levels presumably reflect systemic and inflammatory reactions. In contrast to CRP, PTX3 is directly produced by macrophages involved in arteriosclerosis as well as by vascular endothelial cells, vascular smooth muscles, and neutrophils following stimulation with IL-1 or TNF.
PTX3 is associated with human heart disease and is a potential predictor of acute myocardial infarction. PTX3 level has been shown to increase during the early stages of myocardial infarction. Recent reports have suggested that PTX3 reduces vascular diseases occurring during sepsis and enables early detection of pulmonary hyperemia and is associated with bone metabolism.

References

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Human Pentraxin3 / TSG-14 ELISA System

Human Pentraxin3 / TSG-14 ELISA System

The Human Pentraxin 3/TSG-14 ELISA System can be used to measure PTX3 levels in plasma samples obtained using EDTA blood collection tubes or FBS-free culture supernatant samples in a highly sensitive and accurate manner.

Performance characteristics: Dilution linearity and detection limit

  • Wide measurement range. Can measure concentrations in the range of 0.1–20 ng/mL with accuracy and sensitivity.
  • No cross reactivity with C-reactive protein (CRP) or SAP, which are also pentraxin family members.

Point of Precaution for Samples Suitable for Measurement and Processing of Measurement Samples

Samples suitable for measurement

  • Plasma collected using EDTA -2Na/2K blood collection tubes
  • Fetal bovine serum (FBS)-free culture supernatant

Precaution during sample preparation

  • Serum and plasma samples obtained using heparinized blood collection tubes cannot be used because accurate PTX3 levels cannot be determined.
  • Sample processing ranging from whole blood to plasma separations should be performed within 4 h, and samples should be stored at or below -20°C.
Product code PP-PD03-E0 Spec SDS
Distributor Please see “Distributors”.

Measurement protocol (schematic diagram)

Human Pentraxin3 / TSG-14 ELISA System kit contents

  • Anti-human PTX3 antibody precoated strip well plate, 1 (96 well)
  • HRP conjugated anti-PTX3 antibody reagent, 1vail (12 mL)
  • Human PTX3 standards 0, 0.5, 1.0, 2.5, 5.0, 10, 20 ng/mL, 7 vial (each0.1 mL)
  • 10X Wash buffer concentrate, 1 vial (100 mL)
  • Dilution buffer, 1 vial (15 mL)
  • TMB solution, 1 vial (12 mL)
  • Stop solution, 1 vial (12 mL)
  • Adhesive plate covers, 3 sheets
  • Instruction manual, 1